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| Aerobic Respiration - 91 sec NS201
Ani Galstian
Jane Choi
James Han
Paula Kim
Lab 9: Aerobic Respiration
Description of Video
Our video shows the steps we took to observe aerobic respiration, which consists of the Krebs cycle, and the electron transport chain. We observed this in germinated and dry peas, to see if there is any difference in the amount they respirate. We measured respiration in two ways: by the amount of carbon dioxide being produced, and by the amount of oxygen taken in. In order to measure the amount of oxygen consumed, we added potassium hydroxide (KOH), which absorbs the carbon dioxide being produced during aerobic respiration. Our video shows the amount of respiration undertaken by germinated peas vs. dry peas.
Results and Analysis
Throughout this lab, we observed the amount of carbon dioxide produced and the amount of oxygen taken in by germinated and dry peas during aerobic respiration. The two parts of aerobic respiration are the Krebs cycle and the Electron Transport Chain, which occur in the Mitochondrion. Carbon dioxide is produced during the Krebs cycle, along with NADH, and FADH2, and some ATP. Afterwards, oxygen is consumed during the electron transport chain. Oxygen is the final acceptor of the chain. When it accepts an electron, the electron binds with a hydrogen ion and the oxygen, forming H2O. Thus, the carbon dioxide production happens before the oxygen consumption.
Before measuring our results, we made a few predictions concerning the germinated vs. dry peas. Because dry peas are dormant, we predicted that we would not see evidence of respiration in them. Germinated peas on the other hand are alive, because the enzymes on the outer level of their cells are activated due to the presence of water. Thus, we predicted that we would see clear evidence of respiration.
During the next half hour, we measured the respiration going on in the germinated and dry peas. We did this by the movement of the indicator fluid in the respirometers that were attached to the 4 test tubes, two each of germinated and dry peas. When looking at the results of respiration occurring within the germinated peas, we observed that respiration indeed had taken place. 2.4 cm of oxygen was consumed, and 5.4 cm of carbon dioxide was produced. The carbon dioxide production was 3 cm more than the oxygen consumption. This means that photosynthesis was going on in the germinated peas at a much lower rate than respiration. The consumption of carbon dioxide by photosynthesis was less than the production of carbon dioxide production as a result of respiration. This can be due to the fact that the experiment was conducted in dimly lit room. Next, we observed respiration going on in the dry peas. The oxygen consumption was 0.2 cm, and the carbon dioxide production was also 0.2 cm. The fact that these numbers are equal shows that if any respiration was going on during this time was happening at an equal rate as photosynthesis. However, 0.2 cm is a very small number, and could be a result of error. Our indicator fluid in the respirometer could have moved before we started the experiment, which would make our measurement inaccurate. Thus, we could conclude that respiration did not occur at all.
Through this experiment, we learned about the methods used to measure respiration. We observed the different elements that affect respiration, and cause it to occur. Auteur : choiabin Tags: ns201  |
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